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Journal of Periodontology and Implant Dentistry، جلد ۸، شماره ۱، صفحات ۲۴-۰
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عنوان انگلیسی Assessment of Gingival Fibroblast Attachment to Root Surfaces Restored with Three Different Dental Materials: An in Vitro Study
چکیده انگلیسی مقاله Background and aims. The aim of this study was to compare the biocompatibility of calcium-enriched mixture ce-ment (CEM), composite resin and nano-particled mineral trioxide aggregate (NP-MTA) using human gingival fibroblasts. Materials and methods. A comparative in vitro cell culture study was carried out using 60 single-rooted teeth which were assigned to the following four groups: 1) untreated healthy group (control); 2) restored with composite resin; 3) CEM cement; 4) NP-MTA. The MTT assay was used to measure the viability of fibroblasts attached to each specimen and scan-ning electron microscopy (SEM) was used for describing cell morphology. Results. After 24 hours of incubation, the survival rates for composite resin and NP-MTA were 74.1% and 76.9%, respec-tively, which were significantly lower than the value in the control group, while both were equally biocompatible. No sta-tistically significant difference was found between the control group and CEM cement samples (94.3%). After 3 days of incubation, some increases in the viability of fibroblasts were detected in the composite resin and NP-MTA groups, with their survival rates being 89% and 93%, respectively. Conversely, in the CEM cement group, the survival rate decreased to 80.7%, which was significantly lower than that in the control group (P = 0.0001). Conclusion. The results of in vitro tests indicated that on days 1, 3 and 5 after incubation, composite resin, CEM cement and NP-MTA exhibited acceptable biocompatibility, provided they were allowed to set for 24 hours before exposure to the cells.
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نویسندگان مقاله رضا عمید | reza amid


مریم ترشابی | maryam torshabi


کسری طبری | kasra tabari


مهدی کدخدازاده | mahdi kadkhodazadeh


سارا اسلامی | sarah eslami


میترا قاضی زاده ahsaie | mitra ghazizadeh ahsaie


نشانی اینترنتی http://dentistry.tbzmed.ac.ir/jpid/index.php/jpid/article/view/242
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Research Article
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