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Journal of Dental Research, Dental Clinics, Dental prospects، جلد ۹، شماره ۳، صفحات ۱۳۸-۱۴۳
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عنوان انگلیسی Evaluation of antimicrobial effects of different concentrations of triple antibiotic paste on mature biofilm of Enterococcus faecalis
چکیده انگلیسی مقاله Background and aims. Triple antibiotic paste (TAP) is widely used in endodontics for root canal disinfection, particularly in regenerative procedures. The aim of this in vitro study was to evaluate the antimicrobial effects of different concentrations of TAP at 1-, 2-, 3-, and 4-week intervals on mature Enterococcus faecalis biofilm. Materials and methods. A total of 287 extracted one-rooted human central incisors were infected with E. faecalis ATCC 29212 after removing the crown and preparation. The root canal space was filled with one of the 0.01-, 0.1-, 1-, 10-, 100-, and 1000-mg/mL concentrations of TAP or normal saline (control). The root canal dentin was sampled after 1, 2, 3, and 4 weeks. The dentinal shavings were cultured on Mueller-Hinton agar plates after serial dilutions. The classic colony-forming unit (CFU) counting technique was used to determine remaining bacterial counts. Data were analyzed by using the two-way ANOVA, post hoc Tukey tests and one-way ANOVA (P< 0.05). Results. TAP completely eliminated E. faecalis biofilms at all the intervals at concentrations of 1000, 100, and 10 mg/mL, whereas 1-, 0.1-, and 0.01-mg/mL TAP resulted in significant reduction of CFU means compared with the control group. There were no statistically significant differences between the four time intervals. Conclusion. Use of lower concentrations of TAP at short term could eradicate E. faecalis biofilm and decrease high-concentration side effects.
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نویسندگان مقاله محمد فروغ ریحانی | mohammad frough reyhani


سعید رحیمی | saeed rahimi


زهرا فتحی | zahra fathi


سحر صفورا | sahar shakouie


امین سالم میلانی | amin salem milani


محمد حسین سروش برحقی | mohammad hossein soroush barhaghi


جواد شکری | javad shokri


نشانی اینترنتی http://dentistry.tbzmed.ac.ir/joddd/index.php/joddd/article/view/1757
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/183/article-183-319664.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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